ABSTRACT
Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultured in vitro on MS medium supplemented with IAA, IBA, NAA, 2, 4-D, KN, BAP, TDZ and 2-iP (1.0, 2.0 and 5.0 μM) and BAP/TDZ/KN (1.0, 2.0 and 5.0 μM) in combination with IAA, IBA, NAA, 2, 4-D (1.0, 2.0 and 5.0 μM). The cultured ovules involved in callus induction and an optimum quantity of 93.15 mg of callus from cultured ovules was obtained on the MS medium fortified with 1 μM BAP + 2 μM NAA. The callus induced on medium supplemented with 1 μM BAP+2 μM NAA was subcultured on MS medium supplemented with 5 μM BAP was involved in shoot organogenesis and developed a maximum of 5.55 shoots. The rooting of the regenerated shoots was achieved on MS medium supplemented with 1 μM IAA. Cytological analysis of the root tips of regenerated plants confirmed their haploid nature.
ABSTRACT
This study was concentrated on the production of eleutherosides and chlorogenic acid in embryogenic suspension cultures of Eleutherococcus senticosus by exposing them to different concentrations (50-400 µM) of methyl jasmonate (MJ) during the culture period. In the bioreactor cultures, eleutheroside content increased significantly by elicitation of MJ, however, the fresh weight, dry weight and growth ratio of embryos was strongly inhibited by increasing MJ concentrations. The highest total eleutheroside (7.3 fold increment) and chlorogenic acid (3.9 fold increment) yield was obtained with 200 µM MJ treatment. There was 1.4, 3.4 and 14.9 fold increase in the eleutheroside B, E, and E1 production respectively with such elicitation treatment. These results suggest that MJ elicitation is beneficial for eleutheroside accumulation in the embryogenic cell suspension cultures.